Abstract
The volatile, odorous fraction of neem oil coded as NIM-76 obtained by steam distillation was investigated for in vitro spermicidal activity. The data showed that the minimum concentration which inhibited spermatozoal motility was 0.25 mg/ml for rat and 25 mg/ml for human spermatozoa. The effect of the drug on spermatozoal motility was found to be dose-dependent. The activity of this drug was not altered in the presence of vaginal or cervical mucus. Intra-vaginal application of NIM-76 in rabbits showed no irritation to the vaginal mucosa.
MeSH terms
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Animals
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Cervix Uteri / metabolism
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Chromatography, Gas
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Female
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Gas Chromatography-Mass Spectrometry
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Glycerides / adverse effects
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Glycerides / isolation & purification
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Glycerides / pharmacology*
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Humans
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In Vitro Techniques
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Male
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Mucus / drug effects
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Plant Oils / isolation & purification
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Plant Oils / pharmacology*
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Rats
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Sperm Motility / drug effects
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Spermatocidal Agents / pharmacology*
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Spermatozoa / drug effects*
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Terpenes / adverse effects
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Terpenes / isolation & purification
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Terpenes / pharmacology*
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Vagina / drug effects
Substances
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Glycerides
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Plant Oils
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Spermatocidal Agents
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Terpenes
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neem oil