Objective: To study the chemical constituents from the roots of Rehmannia glutinosa.
Method: The compounds were isolated by various chromatographic methods and identified by spectroscopic analysis.
Result: Twelve compounds were isolated and their structures were identified as 5-hydroxymethyl-pyrrole-2-carbaldehyde (1), 5-hydroxymethyl furfural (2), tyrosol (3), 5,6-dihydroxy-beta-ionone (4), 6-O-E-feruloyl ajugol (5), acteoside (6), leucosceptoside A (7), martynoside (8), isomartynoside (9), purpureaside C (10), jionoside A1 (11), and jionoside B1 (12).
Conclusion: Compounds 1, 3 and 9 were isolated from the genus Rehmannia for the first time.