Fucoxanthin regulates adipocytokine mRNA expression in white adipose tissue of diabetic/obese KK-Ay mice

Arch Biochem Biophys. 2010 Dec 1;504(1):17-25. doi: 10.1016/j.abb.2010.05.031. Epub 2010 May 31.

Abstract

Fucoxanthin, a marine carotenoid found in edible brown seaweeds, attenuates white adipose tissue (WAT) weight gain and hyperglycemia in diabetic/obese KK-A(y) mice, although it does not affect these parameters in lean C57BL/6J mice. In perigonadal and mesenteric WATs of KK-A(y) mice fed fucoxanthin, mRNA expression levels of monocyte chemoattractant protein-1 (MCP-1) and tumor necrosis factor-α (TNF-α), which are considered to induce insulin resistance, were markedly reduced compared to control mice. In contrast to KK-A(y) mice, fucoxanthin did not alter MCP-1 and TNF-α mRNA expression levels in the WAT of lean C57BL/6J mice. Interleukin-6 (IL-6) and plasminogen activator inhibitor-1 mRNA expression levels in WAT were also decreased by fucoxanthin in KK-A(y) mice. In differentiating 3T3-F442A adipocytes, fucoxanthinol, which is a fucoxanthin metabolite found in WAT, attenuated TNF-α-induced MCP-1 and IL-6 mRNA overexpression and protein secretion into the culture medium. In addition, fucoxanthinol decreased TNF-α, inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) mRNA expression in RAW264.7 macrophage-like cells stimulated by palmitic acid. These findings indicate that fucoxanthin regulates mRNA expression of inflammatory adipocytokines involved in insulin resistance, iNOS, and COX-2 in WAT and has specific effects on diabetic/obese KK-A(y) mice, but not on lean C57BL/6J mice.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipokines / genetics*
  • Adipose Tissue, White / drug effects*
  • Adipose Tissue, White / metabolism*
  • Adipose Tissue, White / pathology
  • Animals
  • Anti-Obesity Agents / pharmacology
  • Blood Glucose / metabolism
  • Cell Line
  • Diabetes Complications / blood
  • Diabetes Complications / genetics*
  • Diabetes Complications / immunology
  • Diabetes Complications / pathology
  • Female
  • Gene Expression Regulation / drug effects*
  • Hypoglycemic Agents / pharmacology
  • Macrophages / drug effects
  • Macrophages / immunology
  • Mice
  • Obesity / blood
  • Obesity / genetics*
  • Obesity / immunology
  • Obesity / pathology
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Xanthophylls / pharmacology*

Substances

  • Adipokines
  • Anti-Obesity Agents
  • Blood Glucose
  • Hypoglycemic Agents
  • RNA, Messenger
  • Xanthophylls
  • fucoxanthin