Abstract
The aims of this study were to determine bioactive components of Graviola leaf extracts and to examine the radical scavenging capacity, gene expression and transcription factors of antioxidant enzymes. Rutin, kaempferol-rutinoside, and vitamin U were identified from the steaming and 50% EtOH extracts of Graviola leaves. Graviola leaf extracts effectively scavenged peroxy and nitrogen radicals. 50% EtOH of Graviola leaves provided a 1-2.9 times higher trolox equivalent than the steaming extract. It also had a higher VCEAC. Graviola leaf extracts reduced the generation of reactive oxygen species (ROS) induced by H2O2 in a dose-dependent manner. The 50% EtOH extract of Graviola leaves upregulated SOD1 and Nrf2, but catalase and HMOX1 were not altered by the 50% EtOH extract of Graviola leaves.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Annona / chemistry*
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Antioxidants / metabolism*
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Catalase / genetics
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Catalase / metabolism
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Heme Oxygenase-1 / genetics
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Heme Oxygenase-1 / metabolism
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Hep G2 Cells
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Humans
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Hydrogen Peroxide / metabolism
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Kaempferols / analysis
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Kaempferols / pharmacology
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NF-E2-Related Factor 2 / genetics
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NF-E2-Related Factor 2 / metabolism
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Plant Extracts / pharmacology*
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Plant Leaves / chemistry*
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Reactive Oxygen Species / metabolism
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Rutin / analysis
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Rutin / pharmacology
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Superoxide Dismutase-1 / genetics
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Superoxide Dismutase-1 / metabolism
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Transcription Factors / genetics
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Transcription Factors / metabolism
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Up-Regulation*
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Vitamin U / analysis
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Vitamin U / pharmacology
Substances
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Antioxidants
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Kaempferols
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NF-E2-Related Factor 2
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NFE2L2 protein, human
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Plant Extracts
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Reactive Oxygen Species
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SOD1 protein, human
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Transcription Factors
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Vitamin U
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Rutin
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kaempferol
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Hydrogen Peroxide
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Catalase
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HMOX1 protein, human
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Heme Oxygenase-1
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Superoxide Dismutase-1